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Atelier Ayesha The Alchemist of Dusk follows the story of a girl who has been living alone in a secluded workshop ever since her grandfather died and her younger sister went missing a few years ago. She supports herself by making and selling medicine. As the story begins, she finds out that her younger sister IS alive somewhere in the world. Now, she must devote herself to finding her younger sister. Her journey to keep her promise begins.